Gene description for CD86
Gene name CD86 molecule
Gene symbol CD86
Other names/aliases B7-2
B7.2
B70
CD28LG2
LAB72
Species Homo sapiens
 Experiment description of studies that identified CD86 in Dendritic cells

1
Experiment ID50
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method FACS
PubMed ID 14582906   
OrganismHomo sapiens
Experiment descriptionExosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid.
AuthorsAdmyre C, Grunewald J, Thyberg J, Gripenbä S, Tornling G, Eklund A, Scheynius A, Gabrielsson S
Journal name ERJ
Publication year2003
SampleDendritic cells
Sample nameMonocyte-derived dendritic cells
Isolation/purification methodsDifferential centrifugation
Immunobeads (EpCAM)
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Immunoelectron microscopy
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2
Experiment ID562
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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3
Experiment ID563
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 10K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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4
Experiment ID564
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F3 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.11 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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5
Experiment ID565
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry [LTQ ORBITRAP]
PubMed ID 26858453   
OrganismHomo sapiens
Experiment descriptionProteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
AuthorsKowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Thé C
Journal name PNAS
Publication year2016
SampleDendritic cells
Sample namemonocyte-derived dendritic cells (F5 100K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density1.14 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ ORBITRAP]
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